Vestnik of the Foundation for Fundamental Research, 2010, No.1(51)

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Vestnik of the Foundation for Fundamental Research, 2010, No.1(51)

VESTNIK OF THE FOUNDATION FOR FUNDAMENTAL RESEARCH
Published by RUE "Publishing House «Belaruskaya Navuka»", Minsk, Republic of Belarus

Number 1(51), 2010

THE DAY OF BELARUSIAN SCIENCE

Congratulations by the President of the Republic of Belarus on the Day of Belarusian Science. P. 5

SCIENTIFIC PUBLICATIONS

Serchenya T. S., Sviridov O. V.
The peculiarities of tryptophan residues microenvironment in the tertiary structure of human alpha-1-microglobulin. pp. 6-14

Summary: By means of a fluorimetric analysis of the three-dimensional structure of human alpha-1-microglobulin (A1M), a lipocalin family protein, the microenvironment of the tryptophanyls and their accessibility to solvent molecules were characterized for the first time. Based on A1M tertiary structure computer modeling and the data on protein fluorescence quenching titration with acrylamide and iodide ions, it was concluded that the tryptophan residues of the protein molecule are substantially buried. It was found the fast intramolecular mobility of A1M associated with the fluctuations of protein structure in a nanosecond time range.

Dokukova N. A., Konon P. N., Kaftaikina E. N.
Comparative analysis of three calculation methods of the multibody mechanical vibration systems. pp. 15-23

Summary: The vibro-isolators are used as the power unit suspensions of the motor transport. A new method of series using differential operators to research dynamic characteristics of vibro-isolator is presented. Important analytic dependences between vibration system parameters providing optimal tuning of the machine-building construction vibro-isolator were deduced. These dependences are allowed to manage vibration processes efficiently, to tune increased antihunting domains in necessary frequencies. Comparative analysis was conducted for the three following calculation methods of the multibody mechanical vibration systems: method of the impedance, chain dynamic systems methods and consistent using of the differential operators.

Alexeev G. D., Baturitsky M. A., Davydov Yu. I., Dvornikov O. V., Piskun A. A., Terletskiy A. V., Tokmenin V. V., Tchekhovski V. A., Shulhevich Yu. F.
The semicustom integrated transimpedance amplifier - discriminator microcircuit for gas-discharge detectors. pp. 24-34

Summary: The integrated circuit AD-1.3 consisting of a transresistance amplifier Ampl-1.3 and comparator Disc-1.4 is designed on a specialized analogous Master-Slice Array ABMK_1_3. AD-1.3 possesses large amplification, resistance to self-excitation, high speed (time of leading and trailing edges of amplifier output signal are t_l=4 and t_t=6 ns, respectively, the minimal delay of the amplification/discrimination channel is T_D = 6.2 ns, discriminator output pulse edges are t_l=2.2 and t_t=1.3 ns, respectively, at a load 110 Ohm, and low noise level (I_noise = 24 + 0.39C_D (pF)). The signal-readout electronic module ADB-1 mounted on a four-layer printed-circuit-board 25 x 14 mm has been designed. I can be mounted inside separate drift tubes or inside the end part of multichannel mini-drift tubes (MDT) is developed. ADB-1 has passed tests with a prototype of an MDT muon detector in JINR.

Chyzhyk V. O., Maljush M. K.
Cytomorphological and biochemical characteristics of plants, growing on the higher level of radioecological pressure territories. pp. 35-45

Summary: It was confirmed that in plant genome of different systematic groups, growing in radio-ecological reservation, the changes, dealing with partly not repaired DNA damages and redistribution of its parts, bounded with proteins, changes of cells and nuclei size, changes of pigment system functioning and assimilation apparatus are taking place. Express method of PDN definition in the cell nuclei was elaborated. Radiation differently influences on morphophysiological and biochemical parameters of plants of different systematic groups and ages, which can be the indicator of environment influence.

Bashilov A. V.
Influence lipoperoxids on antioxidizing properties of flavonoid-containing extracts. pp. 46-51

Summary: It is established that extractical substances of leaves, inflorescences, roots and rhizomes of Filipendula hexapetala Gilib. render inhibitorical action on peroxidical oxidation of linseed oil. The received extracts can be recommended as inhibitors peroxidical oxidations of linseed oil with the purpose of prolongation of its period of storage.

Kavalenka V. V., Lamakova V. A., Erchak N. P.
Growth regulating activity of hydrooxalates of methylbis(phenylmethyl)-g-aminopropylsilanes. pp. 52-59

Summary: New water solvable organosilicon compound - hydrooxalate methylbis(phenylmethyl)-g-(N-cyclohexil)aminopropylsilane was synthesized. The growth regulating activity of the hydrooxalate obtained and the hydrooxalates of methylbis(phenylmethyl)-g-piperidinopropylsilane and methylbis(phenylmethyl)-g-pirrolidinopropylsilane described by the authors earlier was studied on radish and lupin. There were determined the concentrations of hydrooxalates solutions which stimulate as well as inhibit germination energy, germination capacity and general length growth of the embryonic roots of the germinating seeds.

Potapovich M. V., Rubinov D. B., Eryomin A. N.
Effect of the herbicides tralkoxydim and sethoxydim on the glucose oxidase Aspergillus Niger oxidation of glucose in the presence of 2,6-dichloroindophenol and potassium ferricyanide. pp. 60-69

Summary: The process of the glucose oxidase (GO) catalysed oxidation of glucose in the presence of 2,6-dichloroindophenol and potassium ferricyanide as the electron acceptors has been optimised for study of potential pesticides influence on GO. The enzyme reaction (30 nM GO) is possible under aerobic conditions and in the citrate-phosphate buffer (CPB), pH 3,5 with 5% dimethylformamide (DMF). The oxygen presence in the solution is reflected on the GO operational stability, which increased with the O₂ and the product of its reduction - H₂O₂ expenditure. The influence of sethoxydim and tralkoxydim on GO at pH 3.5 in systems containing 2,6-dichloroindophenol, potassium ferricyanide and their combination is compared. The greatest influence of the herbicides on GO is observed in system with 2,6-dichloroindophenol. In the enzyme system at pH 3,5 potassium ferricyanide can be used. The effective constants K_м, K_i and k_cat of glucose have been determined for its oxidation in the presence of GO, 2,6-dichloroindophenol and herbicides in 20 mM CPB, pH 3.5 with 5% DMF at 20 °C. Increasing the sethoxydim and tralkoxydim concentration changes the type of the inhibition from the mixed (K_мⁱ > K_м, V_maxⁱ < V_max) up to the unmatched (K_мⁱ < K_м, V_maxⁱ < V_max).

Piven N. V., Orlova E. E., Luchverchyk L. N.
Immunoenzyme assay of autoantibodies to glutamatic acid decarboxylase - a marker of diabetes mellitus. pp. 70-77

Summary: In the paper there are presented data on autoantibodies to glutamic acid decarboxylase (GAD) - highly informative diagnostic and pathogenetic marker of insulin-dependent diabetes mellitus (type I diabetes), presence of which in the blood could be revealed months and years before the appearance of the first clinical symptoms of type I diabetes. The authors have elaborated a new enzyme-linked immunosorbent assay (ELISA) which approbation has been performed on the clinical material. It is substantiated the use expediency of ELISA of autoantibodies to GAD concentrations as clinical-diagnostic, pathogenetic and prognostic marker of type I diabetes at different stages in children and adults.

Kozel N. V., Domanskii V. P., Shalygo N. V.
Photosynthetic apparatus activity and antioxidant system condition in tobacco plants with overexpression of ascorbate peroxidase under photooxidative stress. pp. 78-87

Summary: The features of influence of dye-sensitizer Rose Bengal (RB) an the functional activity and structural organization of photosystem (PS) 2 in gene modified tobacco plants were revealed. Under the photooxidative stress induced by sensitizer RB a destruction of the structural proteins of the PS2, a disturbance of a distribution of the energy between the PS1 and the PS2 as well as inhibition of the PS2 activity were less in the tobacco plants transformed by sence gene of ascorbate peroxidase than in the control plants. This is caused by effective detoxification of the reactive oxygen species in transgenic tobacco plants that have higher the antioxidant status as compared to the control. It has been suggested the assumption that tobacco plants transformed by sence gene of ascorbate peroxidase will be more resistant to any abiotic stress accompanying by accumulation of hydrogen peroxide.

INTERNATIONAL RELATIONS

Protocol on the results of negotiations of the Vietnamese Academy of Science and Technology and the Belarusian Republican Foundation for Fundamental Research. P. 88

BRFFR - DFG. pp. 89-91

COMPETITIONS RESULTS

Joint competition of fundamental research projects "BRFFR-VAST-2010" of the BRFFR and the Vietnamese Academy of Science and Technology. P. 92