Proc. of the Natl. Academy of Sciences of Belarus, Ser. Medical Sci., 2009, No.1
/ Publications / Scientific Journals
Proc. of the Natl. Academy of Sciences of Belarus, Ser. Medical Sci., 2009, No.1
|
Proceedings of the National Academy of Sciences of Belarus
(Vestsi Natsiyanalnai Akademii Navuk Belarusi)
SERIES OF MEDICAL SCIENCESPublished by The Belaruskaya Navuka Publishing House, Minsk, Republic of Belarus |
Number 1, 2009
CONTENTS
CLINICAL AND EXPERIMENTAL MEDICINE
Vlasov A. P., Shubenok D. V., Sereda V. V., Fima D. V., Kravchuk Z. I., Odintsov S. G., Deyev S. M., Martsev S. P.
Recombinant immunotoxin scFv4D5-barnase directed against c-ErbB2-positive breast cancer cells: folding, stability and cytotoxicity. pp. 5--13
Summary: This work provides the structural and functional study of c-ErbB2-targeted recombinant immunotoxin scFv4D5-barnase. The protein was demonstrated to possess well-defined secondary and compact tertiary structure in the pH range 3.0--7.0. The functional activity of the antigen-binding and toxic modules remains unaltered by their fusion into a single chimeric construct. The recombinant immunotoxin scFv4D5-barnase is capable of efficiently binding to the c-ErbB2-expressing cells and exerting the clearly seen cytotoxic effect toward the BT-474 cells that overexpress c-ErbB2.
Kharuzhyk S. A., Chiz G. V., Bogushevich E. V., Gatskevich G. V., Kandybovich D. S., Matskevich S. A., Ugolkova S. A., Bichan Zh. V., Semenov S. V.
Dose levels for computed tomography examinations. pp. 14--22
Summary: Radiation doses from main types of computed tomography (CT) examinations were measured on 8 scanners at health care institutions in Minsk, Vitebsk and Gomel. Mean dose values were determined. Significant variation of dose levels was revealed and explained mainly by difference of scanning parameters. Practicability of introduction into routine use of diagnostic reference levels of dose values for CT examinations was grounded.
Kirillov V. A., Tolstik S. V.
Evaluation of the contribution of mechanically induced paramagnetic centers to the dose load reconstructed by EPR tooth enamel spectra. pp. 23--27
Summary: It is found that the mechanical effect on the teeth produced by dental borers leads to an increased value of a dose measured by EPR spectra of tooth enamel. The evaluated contribution of mechanically induced paramagnetic centers is a necessary condition for adequate estimation of an individual absorbed dose formed due to emergency gamma irradiation.
Shakhbazau A. V., Sevyaryn I. N., Goncharova N V., Kosmacheva S. M., Kartel N. A., Potapnev M. P.
AAV systems for hMSC transduction with neurotrophic and neurogenic factors (CNTF, BDNF, NTF3, noggin). pp. 28--32
Summary: We report creation of expression vectors for neurotrophic and neurogenic factors CNTF, BDNF, NTF3, and noggin, suitable for AAV-mediated delivery into human bone marrow mesenchymal stem cells. Stable expression of these factors, provided by non-pathogenic adeno-associated virus with well-studied chromosome integration site, has the potential to contribute to neuronal differentiation of hMSC.
Chesnov Yu. M., Shved M. M., Bashkevich A. V., Pashkevich D. V., Drozdovsky K. V.
Results of reconstruction of the right ventricle outflow tract and the pulmonary artery in surgical treatment of congenital heart diseases. pp. 33--36
Summary: The article contains the analysis of surgical treatment of 99 patients after right ventricle outflow tract and pulmonary artery reconstruction using xenopericardial patches "Biocard". A control group of patients consists of 90 patients after similar procedures with the use of synthetic materials and glutaraldehyde-treated autologous pericardium. "Biocard" is characterized by optimal physical qualities, high biocompatibility and can be used as material of choice during surgical repair of congenital heart diseases.
Pozdnyakova A. S., Levi D. T., Shpakovskaya N. S., Grichik L. N., Zdanevich I. P., Kondratovich L. V., Rineiski I. N.
TB complex chemical prophylaxis in teenagers and children. pp. 37--43
Summary: The research examining the use of kipferon immunomodulator in 59 children for complex chemical prophylaxis in the conditions of high drag TB mycobacterium resistance and the influence of unfavorable factors (both TB of medical and social character) has been carried out. Thirty children received kipferon alongside with anti-tuberculosis preparations. 29 children were subjected only to anti-tuberculosis preventive therapy. High effectiveness of complex chemical prophylaxis was defined due to a considerable reduction of the tuberculin susceptibility (while applying kipferon) with a valid difference in the results of the experimental group. The impact of unfavourable factors reduces nonspecific resistance of the TB infected and contact and requires complex chemical prophylaxis (anti-tuberculosis preparations with the immunomodulator) influencing two interconnected links of the infectious TB process (micro and microorganism).
Kabak S. L., Kabak Yu. S., Anischenko S. L., Kolb Y. L.
Cell composition of periapical granuloma. pp. 44--50
Summary: On the basis of the analysis of the cell composition of periapical granulomas their tissue heterogeneity, different terms of the beginning of the pathological process and a rate of its development were determined. The obtained data confirm that both humoral and cellular mechanisms of immunity play an essential role in pathogenesis of granulomatous inflammation.
Khodulev V. I., Nechipurenko N. I., Agaryshev A. A.
Electroneuromyographic characteristic of peripheral nerves at weak chronic lead intoxication. pp. 51--56
Summary: Based on the clinical data it is found that the symmetrical distal polyneuropathy with involving autonomic and sensory nerves of the lower extremities develops under weak chronic lead intoxication. The use of electroneuromyography has shown that along with this the motor fibers and nerves of the upper extremities are involved. At lead intoxication an inconsiderable change in the form of the M-response is revealed. This is characteristic of the uniform lesion of motor fibers. It is found that the amplitude of M-responses and the sensory nerve action potential decrease and the conductive function of peripheral nerves undergoes fault. Thus, the results obtained show that at mild chronic lead intoxication there develops the primary ax-onal process, which results in the lesion of peripheral nerves and the fault of their conductive function.
Shilov V. V., Kozmidiadi A. O., Nadina N. G., Mashkovich A. E., Tumar E. M.
Neoton effect on the cardiovascular system and the physical performance of laboratory animals. pp. 57--60
Summary: It is established that neoton studied in the range of doses from 10, 50 to 250 mg/kg does not stimulate the performance of experimental animals both under short and long-term infusion. When using the preparation for 4 weeks in a dose of 250 mg/kg the physical endurance decreases. The heart rate and arterial pressure after neoton infusion in doses of 100--300 mg/kg do not change. The drug has no vasodilatation and anti-hypoxic properties.
Asadchuk T. V., Mosse K. A.
Polymorphism of the CTG-repeats in the myotonin protein kinase gene in the population of Belarus. pp. 61--65
Summary: The mutation specific for myotonic dystrophy is an unstable expanded CTG-repeats located in the 3'-untranslated region of the myotonin protein kinase gene. The objective of this study was to determine the polymorphism of CTG-repeats in the population of Belarus. In 200 chromosomes we found 15 different allelic variants from 5 up to 28 CTG-repeats. The allele frequency distribution had two peaks corresponding to the alleles with 5 and 11--14 CTG-repeats. The most common alleles had 5 repeats (38%). The heterozygosity of the CTG polymorphism in the population of Belarus was established as much as 78%, with only 22 homozygotes detected. So, this method using direct PCR analyses is very efficient as the first stage of myotonic dystrophy diagnosis.
Khomich T. I., Pronko P. S.
Effect of N-acetylcysteine and lipoic acid on the state of antioxidant system in the rat liver in ethanol withdrawal. pp. 66--69
Summary: Changes in the antioxidant system activity typical for oxidative stress were found during the postintoxication period following the 5-day intragastric administration of ethanol (10 g/kg body weight) to Wistar rats. The administration of lipoic acid (40 mg/kg), N-acetylcysteine (100 mg/kg) or their combined administration changed the metabolic response to the alcohol-induced oxidative stress exhibited in the inhibition of a lipid peroxidation rate in alcohol-treated animals. Under experimental conditions, the substances tested effectively enhanced the activity of antioxidant protection in the rat liver during the ethanol withdrawal following massive alcohol intoxication.
Soltanov V. V., Sergeyev V. A., Komarovskaya L. M.
Changes in the transit of the colon content and its electrical activity of experimental colitis. pp. 70--76
Summary: In acute experiments on rats (urethane anesthesia, 110 mg per 100 g body weight), it was found that the dye Evans blue applied into the cecum spread in the colon for 42 ± 4% of its length and for 67 ± 5% (P < 0.05) at 3--4 days of experimental colitis induced by 1 ml of 4% acetic acid administered into the descending colon. On the basis of these data and the results of recording the colonic potentials in similar experiments it is suggested that the transit acceleration and development of diarrhea are due to the enhanced stimulatory reflex influences of cecal receptors on the colon.
Rutkevich S. A., Karavai T. V., Chumak A. G.
Activation and depression of "silent" efferent sympathetic neurons impulse activity in viscero-visceral and somato-visceral reflex circles. pp. 77--84
Summary: The group pertaining and the electrostimulation mode of n. mesentericus, n. saphenus and n. tibialis afferent fibers were determined in anaesthetized rats. Simultaneous electrostimulation of these nerves caused the activation or depression of "silent" sympathetic efferent neurons forming pl. aorticus abdominalis. It was assumed that 1--50 Hz frequencies stimulation of visceral C-afferents provoked the phasic sympathetic neurons activation due to the "wind up" phenomenon. The activation resulted in the potentiation of efferent sympathetic neurons impulsation. Somatic Àgd (threshold 0.3--0.6 B) or Àgd+C (threshold 1.0 B) afferent fibers electrostimulation led to the depression of the phasic but not tonic sympathetic efferent activation.
Skrahina A. M.
Peripheral blood and broncho-alveolar lavage fluid cytokins in multidrug resistant tuberculosis. pp. 85--92
Summary: A good understanding of the host immune response to the strains of Mycobacterium tuberculosis (Mb. t.) with resistance to drugs is therefore important to prevent the disease or to develop new treatment. Clinical interpretation of levels and functions of cytokines in tuberculosis (TB), and in multidrug resistant (MDR), TB in particular is quite difficult so far. In the present study we propose the evaluation of the levels of IFN-g, TNF-a, IL-1, IL-4, IL-8, G-CSF in peripheral blood and broncho-alveolar lavage fluid (BAL) in patients with MDR and drug susceptible (DS) TB at the early stage of the disease. 17 patients with MDR TB and 20 patients with DS TB were examined at the time of hospital admission. 16 healthy individuals were a control group. The levels of IFN-g, TNF-a, IL-1, IL-4, IL-8, G-CSF in peripheral blood and BAL were measured by enzyme-linked immunosorbent assay (ELISA). As compared to DS TB, a significant reduction of the blood level of IFN-g, IL-4, G-CSF was found in MDR TB. There was also a significant decrease in TNF-a BAL concentration in patients with MDR as compared to DS TB. The results of our study suggest that at the early stage of the disease resistant to drugs strains of Mycobacterium tuberculosis are able to induce some depression of the host immune response to these pathogens.
Povelitsa E. A., Nadyrov A. A.
Estimation of the concentration of insulin-like growth factor I (IGFI) and insulinlike growth factor-binding protein (IGFBP3) in the blood serum of patients with different forms of prostate cancer. pp. 93--97
Summary: The growth factors in the blood serum of patients with prostate cancer were studied. The concentration of insulin-like growth factor I (IGFI) and insulin-like growth factor-binding protein (IGFBP3), the level of reproductive sex hormones, total and free prostate specific antigen (PSA) levels in the blood serum of patients with local and metastatic forms of prostate cancer were determined before conducting special treatment, and also in the control group. The enzyme multiplied immunoassay in combination with the standard methods was used for examination of such patients. The results obtained show that the concentration of IGFI and IGFBP3 in the blood serum of patients with prostate cancer differs from that of healthy ones. Furthermore, the correlation of IGFI and its binding protein IGFBP3 concentration with the level of reproductive sex hormones in the blood serum, and also with the fundamental clinical-morphological characteristics of prostate cancer was established. It was shown that the tendency towards a change in the level of IGFI and IGFBP3 concentration in the blood serum of patients with first revealed prostate cancer depends on the presence or the absence of the distant metastases.
Mastitskaya S., Khulup G., Denecke B.
Biomaterials as three-dimensional scaffold for culturing and differentiation of cells aimed at cardiomyoplastics. pp. 98--100
Summary: The influence of biomaterials on the human mesenchymal stem cells (MSCs) cardiac differentiation in vitro was studied. The biomaterials RG503 and Texin 950 were found to be the most promising scaffolds for cardiac-like cells generation from hMSCs in vitro since the hMSCs cultured on these biomaterials demonstrated the highest level of cardiac-specific genes (myosin heavy chain and transcriptional factors Nkx2.5 and MEF2D) expression.
Konoplya N. E.
Role of the high dose chemotherapy with autologous peripheral stem cell transplantation in the treatment of medulloblastoma in children. pp. 101--105
Summary: 31 children with morphologically verified diagnosis of medulloblastoma high risk group received high dose chemotherapy with autologous peripheral stem cell/bone marrow transplantation as the first-line therapy. 6-year RFS rate was 0.77 ± 0.08 (Me = 31.03 months) and OS was 0.83 ± 0.07 (Me = 32.97 months) for the whole group of patients. Parts of the patients have not received high dose chemotherapy which allowed us to evaluate its role. The data we have obtained clearly show the efficacy of the high dose chemotherapy. Thus, 6-year RFS was higher in children who had received high dose chemotherapy versus children who had not received it (0.77 ± 0.08 and 0.46 ± 0.10, respectively (P < 0.041)). Thereby, introduction of the high dose chemotherapy with autologous peripheral stem cell transplantation as a new element in the protocol guarantees high efficacy against disease recurrence in the high risk group patients.
SURVEYS
Rotanova T. V., Melnikov E. E.
Energy-dependent proteolysis as a mechanism for keeping the cell proteome. pp. 106--115
Summary: The article characterizes the main enzymatic systems of selective proteolysis belonging to the AAA+ ATPase superfamily and serving for keeping cell proteome in prokaryotes, eukaryotes and archaea. The peculiarities of proteolytic components of the ATP-dependent proteases as well as the similarity and diversity of their regulatory ATPase components are discussed.
Volykhina V. E., Nekhai A. S., Furs H. O.
L-carnitine: structure, properties, application. pp. 116--120
Summary: L-carnitine is a biologically active vitamin-like substance. In order to satisfy the organism's physiologic need for alimentary and endogenous L-carnitine its additional sources are necessary. L-carnitine is a component of biologically active supplements and vitamin complexes. It is used with preventive purposes and as remedy. The high performance of L-carnitine preparations in the complex therapy of different pathological conditions in adults and children is shown. L-carnitine is included into the composition of energotropic complexes at the prescription of metabolic correction of the mitochondrial disorders.
SCIENTISTS OF BELARUS
To the memory of Academician Feodor Vasilievich Oleshkevich. p. 121
Click here to read this article in Russian (Win1251)
/ Publications / Scientific Journals / Back to Top of Page
Designed and maintained by Dr. Nikolai N. Kostyukovich. Last updated: June 10, 2009
Created with assistance of Dr. Ignatii I. Korsak
Copyright © 2009 The National Academy of Sciences of Belarus
Copyright © 2009 The Belaruskaya Navuka Publishing House 
