Proc. of the Natl. Academy of Sciences of Belarus, Ser. Biological Sci., 2010, No.1
/ Publications / Scientific Journals
Proc. of the Natl. Academy of Sciences of Belarus, Ser. Biological Sci., 2010, No.1
| Proceedings of the National Academy of Sciences of Belarus
(Vestsi Natsiyanalnai Akademii Navuk Belarusi)
SERIES OF BIOLOGICAL SCIENCESPublished by The Belaruskaya Navuka Publishing House, Minsk, Republic of Belarus |
Number 1, 2010
CONTENTS
ARTICLES
Rupasova J. A., Volotovich A. A., Vasilevskaya T. I., Pavlovski N. Â., Yakovlev A. P., Pyatnitsa F. S., Pinchucova Yu. M.
The genotipics distinctions of biochemical composition the introducents of fruits of Vacciniaceae in the conditions of Belarus. pp. 5--11
Summary: Interspecific distinctions of quantity indicators of biochemical composition of fruits of a blueberry (V. covilleanum Butkus et Plishka), foxberry (V. vitis-idaea L.) and large cranberry (Oxycoccus macrocarpus (Ait.) Pers.) on 32 indexes are positioned. Taxons of each kind the most perspective for dividing into districts and selection on nutrient and vitaminic value of fruits in the conditions of Belarus are revealed.
Urbanovich O. Yu., Kazlouskaya Z. A., Khatskevich A. A., Kartel N. A.
The analysis of polymorphism SSR-locus of kinds MALUS. pp. 12--17
Summary: The level of genetic differentiation among 40 apple-tree accessions was investigated with SSR markers previously identified in Malusxdomestica Borkh. In total, 306 polymorphic alleles were detected by the 20 SSR markers with an average number of alleles of 15.6 per marker. Polymorphism information content (PIC) varied from 0.73 to 0.94 with an average of 0.87. Totally 40 genotypes of apple-tree cultivars and species were identified by means of these markers. Low significance of species distribution in clusters was noted based on allele analysis of 20 SSR-loci.
Lemesh V. A, Guzenko Ye. V., Yemets A. L, Baer G. Ya., Shisha E. N, Blume Ya. Â., Kartel N. A.
Development of genetically modified plants of fiber flax (Linum usitatissimum) by bioballistic transformation method. pp. 18--23
Summary: The protocol of bioballistic transformation developed by us as well as the method of rhizogenesis stimulation has allowed production of fiber flax model transgenic plants of 2 cultivars (Vasilek, Start) carrying the genetic construction GFP-TUA6. Insertion of chimaeric gene was verified by PCR. Six plants have passed adaptation under ex vitro conditions. Seeds of 3 transgenic self-pollinated lines of cv. Vasilek T1 and those of one transgenic self-pollinated lines of cv. Start T1 were produced. The developed techniques may be used in agricultural biotechnology for producing flax plants with inserted target genes.
Lisovskaja V. M., Pavlyichuk N. V., Voronkova E. V., Yermishin A. P.
Stability to the X-virus diploid interspecific hybrids between Solanum acaule Bitt. and diploids of S. tuberosum. pp. 24--29
Summary: Two year trials of diploid hybrids between X-virus resistant specimen Ñ 46-3 of wild allotetraploid potato species S. acaule and susceptible S. tuberosum dihaploids (Fl, BC1, BC2) made it possible to select the set of forms which can be used in breeding as donors of genes of X-virus resistance. It was revealed rather high efficiency of introgression of X-virus resistance genes of wild species into diploid breeding material in spite of the loss of one genome of wild species during the production of diploid interspecific hybrids.
Galinousky D. V.
Sequencing of the bacterial phytoene synthase gene and comparing with psy-gene of plants. pp. 30--34
Summary: The crtB-gene full length sequence from crt-cluster of Pantoea agglomerans 206 was obtained. On the basis of comparison the translated sequence with that from data bank the structural difference between the crtB-gene product and phytoene synthase of plants were demonstrated. This case makes it possible to consider the bacterial gene a potential transgene for expression in plant organism.
Pomasanov N. N.
Influence of marriage radius on the some phenotype features of city youth of the central Belarus. pp. 35--38
Summary: Features of variability of phenotype signs of descendants from iso- and heterolocal marriages are analyzed. Despite of similarity phen pools two groups, some distinctions on separate phenotypses are marked.
Davidovskii A. L, Veresov V. G.
Binding determinants of interactions between antiapoptotic proteins Bcl-2, Bcl-xL, Mcl-1 and gossipol. pp. 39--41
Summary: The computational docking approach was employed to establish the structural determinants of inhibition of antiapoptotic proteins by anticancer agent gossypol The results obtained suggest that inhibition of antiapoptotic proteins of Bcl-2 family by gossypol is caused by the insertion of this compound into the hydrophobic grooves of antiapoptotic proteins Bcl-2, Bcl-xL and Mcl-1 and by the formation of two hydrogen bonds of gossypol with Asn and Arg of the signature region NWGR of these proteins as well as by an additional hydrogen bond either with lysine (Lys236) of Mcl-1 or tyrosine (Tyr201 of Bcl-2 and Tyrl95 of Bcl-xL). This results in close values of binding energies of these proteins to gossypol with fairly lower ones in the case of Mcl-1. Less significant affinity of gossypol with Bcl-2 or Bcl-xL as compared to that of ABT737 is explained by a hydrophobic effect due to an incomplete occupancy of the groove by gossypol as compared to the case of ABT737.
Yaronskaya E. Â., Shalygo N. V.
The activity of antioxidant system in albino tissue of streptomycin-treated barley seedlings. pp. 42--48
Summary: The activity of antioxidant system in albino tissue of barley seedlings treated at a stage of seeds with streptomycin, an inhibitor of plastid protein synthesis, was investigated. In the white tissue of etiolated and light-exposed seedlings, a considerable increase of the activity of catalase, ascorbate peroxidase, glutathione reductase as well as of the content of cytosolic superoxide dismutase was demonstrated. The albino tissue showed also an essential decrease in the content of total and reduced ascorbate, gluthatione, tocopherol, and in the ratio GSH/GSSG. These results indicate the activation of antioxidant system in the plants treated with antibiotic. The total reactive oxygen species (ROS) content in the white tissue remained similar to control values indicating an effective control of ROS homeostasis in albino tissue. It is supposed that activation of antioxidant system in the white tissue of the plants exposed to streptomycin is caused to modified expression of the nuclear genes encoding its components in response to suppression of generation of plastid signal derived from plastid translation system.
Savchenko G. E., Kabashnikova L. E, Makarov V. N, Strzalka K, Klodawska K, Dubovets N. I.
Effect of heat stress on content of carotenoid pigments in etiolated seedling of hexaploid triticale with different types of intergenomic chromosome substitutions. pp. 49--52
Summary: Changes in carotenoid composition under heat shock (HS, 420C, 3 h) in leaves of etiolated seedlings of hexaploid triticale with different types of intergenomic chromosome substitutions were investigated. New data on changes in xantophyll content in etiolated seedlings under temperature stress were obtained. Metabolic heterogeneity of the violaxanthin pool and a different character of stress response of seedlings at the xanthophylls level has been detected. Owing to this fact the investigated genotypes can be divided into two groups according to the initial content of violaxanthin as well as to the degree of violaxanthin de-epoxidation. Thus, the first group (the lines WRAHS-1, WRAHS-2 and WRAHS-3) was characterized by lover relative content of violaxanthin in the control lines combined with a higher degree of its de-epoxidation. In the second group (the lines WRAHS-4, WRAHS-5 and WRAHS-6), a higher initial content of violaxanthin but approximately two times lower degree of de-epoxidation in comparison to the first group was revealed. Distinctions of carotenoid interconversions are discussed as an important selection criterion of triticale seedling sensitivity to HS.
Melnicov S. S., Manankina E. E., Budakova E. A., Samovich T. V.
Influence of exogenous phytohormones on the growth and contents of pigments in biomass of Spirulina. pp. 53--57
Summary: It was shown, that indolilacetic and gibberellic acids, 6-benzylaminopurine, aresine and phenylurea increase the growth of Spirulina and contents of provitamin A, phycocyanin and chlorophyll which there are the most valuable substances of biomass.
Mikhailova R. V., Semashko T. V., Zhukouskaya L. A., Lobanok A. G.
Application of ultrafiltration technique for concentration of Penicillium funiculosum glucose oxidase. pp. 58--61
Summary: Application efficiency of ultrafiltration membranes of diverse selectivity and hollow-fiber filter elements manufactured in Belarus, Russia, Germany for concentration and desalination of Penicillium funiculosum cultural filtrate containing glucose oxidase was studied. It was shown that technology of producing enzyme preparation glucose oxidase of Penicillium funiculosum at the stage of ultrafiltration concentration required application of units equipped with polyestersulfonic membrane cassettes (PALL, Germany) or polymeric membrane based on aromatic poly sulfonamide UPM 10 ("Vladipor", Russia) ensuring optimal productivity of the process, high glucose oxidase selectivity and the lowest active protein losses.
Tsirkunova Zh. E, Khomich M. Â., Mikhailova R. V., Lobanok A. G.
Screening of mycelia fungi-producers of chitinolytic enzymes. pp. 62--64
Summary: Ability of 113 fungal strains belonging to 15 genera to synthesize extracellular chitinolytic enzymes was studied. It was shown that 63% of tested cultures synthesized extracellular chitinases, and 61% - extracellular chitosanases. Synthesis of chitinolytic enzymes was typical for fungi representing genera Aspergillus, Beauveria, Chaetomium, Cladosporium, Gliocladium, Mortierella, Penicillium, Paecilomyces, Scopulariopsis and Trichoderma. Performed screening allowed to select promising producers of extracellular chitinases - Paecilomyces lilacinus F-2 and Paecilomyces marquandii.
Burko D. V., Kvach S. V., Zinchenko A. I.
Optimization of expression conditions of lysyl-tRNA-synthetases in Escherichia coli recombinant strains. pp. 65--69
Summary: New recombinant strain-producers of E. coli and T. termophilus modified lysyl-tRNA-synthetases (LysU and LysRS) were constructed. The proteins have N-terminal fusion polyhistidine peptide, that allows simple purification by metal affinity chromatography. For the first time it is shown that LysRS T. termophilus can be used for synthesis of diadenosine tetraphos-phate. The optimal conditions for induction of recombinant lysyl-tRNA-synthetases are studied. It is established that the most convenient and cheap way of the recombinant proteins induction is the autoinduction method.
Rakhuba D. V., Novik G. I., Kanterova A. V., Szwajcer-Dey E.
The use of extracts of barley spent grain fractions as a basis of nutrient medium for growth of lactic acid and bifidobacteria. pp. 70--75.
Summary: The possibility for using the barley spent grain fractions as a basis for nutrient media for growth of lactic acid and bifidobacteria was shown. For improvement of growth potential it is reasonable to modify the nutrient media by adding lactose, ascorbic acid, yeast extract and mineral salts. Using modified nutrient media for microorganisms cultivation intensive growth was observed which characterized by high biomass yield, cell viability and acidification activity.
Konoplya E. E, Mironov V. P., Zhuravkov V. V., Kudina O. P.
The modeling of thyroid irradiation in conditions of iodine deficiency at nuclear accidents. pp. 76--79
Summary: The standard Riggs model has been used to calculate the thyroid radioiodine content, the dose factors, and the collective thyroid irradiation doses of the adult population from the Brest area. In conditions of stable iodine receipt deficiency, the dose factors at thyroid gland receipt of 131I were predicted to be 2,5 times higher than under normal receipt of stable iodine. Therefore an actual iodine deficiency should be taken into account upon calculations of thyroid irradiation doses.
Sholukh A. M., Chaly Y. V., Pashkova O. L., Voitenok N. N.
Site-directed and random PEGylation of Fab-fragments of the monoclonal antibody against human tumor necrosis factor. pp. 80--85
Summary: PEGylation is the major method for modification of therapeutic proteins for reducing immunogenicity and increasing time of circulation in the blood. Reaction of polyethylene glycol coupling to protein molecule may lead to loss of activity therefore the development of an effective scheme of PEGylation is extremely important. PEGylation of Fab-fragments of the 5N antibody against human TNF-a by differently activated PEG derivatives was investigated. It was shown that conjugates of 5N fragments with PEG retained functional activity and neutralized cytotoxic action of TNF-a on the cell line L-929. Effective neutralizing concentrations (ED50) were 15 ng/ml for antibody, 5ng/ml for Fab-fragments and lO ng/ml for Fab-PEG. Random PEGylation with yield of PEGylated product about 40% was more effective than site-specific (yield about 10%). Circulation time of randomly PEGylated Fab-fragments in mouse blood was comparable to that of the whole antibody and PEGylated Fabs were detectable during 12 days after injection. The whole set of experimental procedures of the influence of PEGylation on the functional activity of antibody fragments could be recommended as a prototype for the experimental selection of PEGylation type choice.
Perevolotskaya T. V.
Assessment of 137Cs and 90Sr radionuclides as they are involved into biological cycle by the scots pine (Pinus silvestris) upon different levels of ground waters. pp. 86--90
Summary: The following article presents the results of 137Cs and 90Sr assessment as they appear to be involved in a biological cycle being under the effect of pine afforestation and depending on the level of ground waters. According to the study, during a litterfall period of the assimilating organs the annual return of 137Cs makes up 0,1-0,5% out of its total amount in biogeocenosis, and that of 90Sr - up to 0,6-2,3% respectively. Here the lowest 137Cs values are observed on the dry study areas, while the minimum value of 90Sr, by contrast, are found on a well water-logged area. The minimum measured values of activity concentration of 137Cs and 90Sr in transpiration moisture are typical to be observed on waterless areas, the maximum levels of radionuclides activity, however, appear on the overwatered areas of the study.
Litjak V. V., Butrim S. M., Moskva V. V.
The morphological characteristic natural starch a various botanical origin. pp. 91--99
Summary: It is investigated morphological structures natural starch a various botanical origin: potato, corn, tapioca, wheaten, rice, rye, pea, amaranth, barley, red leoti, triticale, chick pea. It is established, that the morphological structure of particles natural starch in the strong depends on a source of a botanical origin of starch and can change in enough wide limits: there are both small, and average and big granules, for example, granules amaranth starch have the sizes of all about 1 micron while granules of potato starch reach 60 microns; the form of granules is not less various also: correct and wrong oval, wrong many-sided, roundish, etc.
Ryzeuski V. K., Pljuta M. V., Leshchenko A. V., Yermalayeva I. A.
New fish species in the ichthyofauna of Belarus - tubenose goby Proterorhinus marmoratus. pp. 100--103
Summary: Morphometric data of tubenose goby Proterorhinus marmoratus (Pallas, 1814) first recorded in the fauna of Belarus are given.
Shlyakhtenok A. S.
Wasps of family Tiphiidae, Scoliidae, Sapygidae, Chrysididae, Mutillidae (Hymenoptera, Aculeata) of Polesie Radiological Nature Reserve. pp. 104--110
Summary: For the period of researches 1989-2000 in 10 biotopics Polesie Radiological Nature Reserve with the help of Malaise traps 2 309 specimens of Tiphiidae, Scoliidae, Sapygidae, Chrysididae, Mutillidae, which belong to 43 species, were collected. The most numerous species were Trichrysis cyanea (157,7 specim/trap-seson), Chrysis angustula (72,3), Chrysis fulgida (53,8), Chrysis ignita (35,4), Chrysis fasciata (17,3), Chrysis mediate (14,6), Pseudomalus auratus (12,9) è Omalus aeneus (12,3) (fam. Chrysididae); Tiphiafemorata (50,7) è Methocha ichneumonides (12,9) (fam. Tiphiidae); Sapyga quinquepunctata (18,9) è Sapygina decemguttata (11,3) (fam. Sapigidae) è Myrmosa atra (15,6) (fam. Mutillidae).
Giginyak I. U.
The characteristic of Caddifly (Tricoptera) fauna in polytypic reservoirs in the territory of Belarus. pp. 111--113
Summary: The 73% from 80 investigated species of Caddisfiy larvae (Trichoptera) are noted for one or two similar types of water objects, 27% possess wider ecological plasticity was shown. The quantity of the presented species and families decreases among following types of water objects: river -- lakes -- time reservoirs -- reservoirs -- streams -- springs -- channels. Families Sericostomatidae, Brachycentridae, Hydroptilidae were found only in rivers, and family Ecnomidae -- only in time reservoirs.
SHORT COMMUNICATIONS
Bashilov A. V., Spirydovich E. V., Tsimafeyeva V. A.
Biochemical structure and pharmacological properties Begonia erythrophylla. pp. 114--116
Summary: The chemical composition of some secondary metabolits Begonia erythrophylla Neumann is studied. It is received medicine "Begonefril" on the basis of leaves of a Begonia erythrophylla Neumann.
Shimalov V. V.
The distribution and the helminth fauna of white-toothed shrew (Crocidura leucodon Hermann, 1780) in Belarus. pp. 117--119
Summary: For the first time the information about the helminth fauna of white-toothed shrew was presented in the Republic of Belarus. 7 white-toothed shrews were investigated in south-west Belarus and with 5 animals 5 species of helminths were found: 3 trematode species and 2 cestode species. Cestode Hymenolepis uncinata (Stieda, 1862) have been registered more often. The information about the localities and the dates of discovery of this rare species of insectivore mammals for Belarus was presented.
SCIENTISTS OF BELARUS
Leonid Michailovich Sushchenya (To the 80th Anniversary). pp. 120-122
Click here to read this article in Russian (Win1251)
/ Publications / Scientific Journals / Back to Top of Page
Designed and maintained by Dr. Nikolai N. Kostyukovich. Last updated: February 19, 2010
Created with assistance of Dr. Ignatii I. Korsak
Copyright © 2010 The National Academy of Sciences of Belarus
Copyright © 2010 The Belaruskaya Navuka Publishing House
