Proc. of the Natl. Academy of Sciences of Belarus, Ser. Biological Sci., 2008, No.3
/ Publications / Scientific Journals
Proc. of the Natl. Academy of Sciences of Belarus, Ser. Biological Sci., 2008, No.3
| Proceedings of the National Academy of Sciences of Belarus
(Vestsi Natsiyanalnai Akademii Navuk Belarusi)
SERIES OF BIOLOGICAL SCIENCESPublished by The Belaruskaya Navuka Publishing House, Minsk, Republic of Belarus |
Number 3, 2008
CONTENTS
ARTICLES
Sergeichik S. A., Sergeichik A. A.,Borsouk E. A.
Ecological and physiological evaluation of formaldehyde influence on the assimilation organs of woody plats. pp. 5--11
Summary: It have been established that formaldehyde leads to the changes of the concentration of chlorophyl a, b and carotinoids as well as photosynthetic pigment ratio, content of protein and activation of peroxidase in leaves of woody plants. 3 groups of plant species were indicated: 1 -- tolerant, 2 -- middle tolerant, 3 -- low tolerant to formaldehyde. Plant species with high level of tolerance to formaldehyde were the most promising for the environment optimization.
Borodich G. S.
Comparative evaluation dwarf cultivars and forms of Iris x hybrida hort. and selection of the ones most suitable for production assortment. pp. 12--15
Summary: It was presented the quotes the variety evaluation of the dwarf varieties and the forms of Iris x hybrida hort. The results of the comparative evaluation 13 varieties and 10 forms of the Iris x hybrida hort after considering are recommended for broad cultivation in the republic.
Pavlovski N. B.
Influence of the type of soil substratum and its temperature condition on the regeneration properties of green cuttings. pp. 16--19
Summary: Regeneration properties of Vaccinium x covilleanum cv. Earlyblue, Bluecrop and Coville green cuttings were defined mainly by the cultivar features. It was found they not depending from the type of substrate applied, and its temperature conditions.
Parfenov V. I., Belomesyatseva D. B.
The mycobiota peculiarities in consortium of the coniferous plants depending on inconsort distribution. pp. 20--28
Summary: Domination of the species with holarctic and palaearctic natural habitats was ascertained. The dependence between mycobiota structure and plant-host areal features was displayed. The following facts are given: the migration of European species to Belarus territory, the primary micromycetes development on strange coniferous plants and their subsequent identification on native coniferous species.
Dubovik D. V.
Adventive component of flora of east part of Belarus. pp. 29--33
Summary: The adventive component of flora of eastern part of Belarus is studied, which is analysed on the row of parameters. It is set that adventive species have a different geographical origin, penetrated on this territory mainly in XIX and XX ages.
Urbanovich O. Yu., Buloichik A. A., Voluevich E. A., Kartel N. A.
Molecular-genetic analysis of alloplasmic wheat lines (T. aestivum L.). pp. 34--39
Summary: Complete genome substitution in alloplasmic wheat lines was studied by markers for micro satellite sequences. Alloplasmic lines were produced on the basis of 7 lines containing cytoplasm of the species Ae. speltoides, T. boeoticum spp. aegilopoides, T. polonicum, Ag. intermedium, Ae. sharonensis, T. turanicum, T. aestivum and nuclear genome of T. aestivum, cv. Penjamo 62. The lines were saturated as a result of a series of backcrosses by a nuclear genome of cvs. Gabo, Lee, Crim and isogenic lines of cv. Thatcher with genes Lr23 and Lr10. Twenty-four SSR loci located on 16 chromosomes of wheat genome were considered. A fragment of genome recipient was detected in two lines. Substitution of recipient genome by genome of appropriate donor was observed in other alloplasmic lines. Inheritance of donor genome was noted in both the lines subjected to 12 backcrosses and the lines produced as a result of 6 backcrosses.
Shakhbazum A. V., Zgurskaya N. A., Kartel N. A., Goranov V. A.
Non-viral transgene delivery into primary rat neuronal and pancreatic tissues. pp. 40--43
Summary: A simple and efficient vector delivery method by using of PEI-based reagent was adapted for various rat primary cell populations. Transfection efficiency of primary DRG neurons was up to 10%, with transfected cells actively participating in secondary nods formation. Transfection of isolated pancreatic b-cells and floating islets provided efficient and prolonged GFP expression in up to 60% cells. Expression of CD59, as well as the other trophic and cytoprotective factors in primary neuronal and pancreatic tissues may improve xenogenic graft survival on early post-operational stage.
Savina N. V., Dalivelya O. V., Kuzhir T. D., Gradska I.
The comparison of two sublines of mammalian cells by the level of endogenous and radiation-induced genome damage. pp. 44--48
Summary: The comparative characteristics of two murine lymphoma sublines L5178Y (LY-R and LY-S) by cell survival and proliferation, the level of endogenous and X-ray-induced DNA double strand breaks and micronuclei were presented. It was shown that irradiation to decrease cell survival and to inhibit their proliferation in the subline LY-S. The frequency of endogenous DNA damage was higher, and repair efficiency of damaged DNA was lower in the subline LY-S as compared to LY-R. The frequency of spontaneous and X-ray-induced micronuclei also significantly increased in the subline LY-S. Thus, it was revealed higher radio-sensitivity of the subline LY-S due to deficient repair of DNA double strand breaks.
Grinev V. V., Maximova N. P., Heidenreich O.
The development of inducible RNA interference system for hybrid AML1/ETO gene expression control. pp. 49--54
Summary: Anti-AML1-ETO artificial microRNA coding DNA insert was successfully synthesized and cloned. This insert was used to design of the pJSATIR/amiAML1-ETO lentivirus transfer vector that provides of intracellular artificial microRNA expression under control of tetracycline/doxycycline inducible bidirectional minimal cytomegalovirus promoter. Next, Kasumi-1 acute myeloid leukemia cells were genetically modified by pJSATIR/amiAML1-ETO lentivirus transfer vector and it was shown that artificial microRNA can downregulate of the aml1-eto gene expression. Moreover it was found that artificial microRNA-mediated depletion of AML1-ETO hybrid protein decreases of proliferative activity and clonogenic properties of Kasumi-1 cell line. Thus lentivirus vector-based system for inducible RNA interference was developed. This lentivirus system can be used in future in vitro as well as in vivo functional investigations of aml1-eto gene.
Pundik A. N., Gapeeva T. A., Tretyakova T. G, Volotovski I. D.
The toxicity for the E. coli and A. tumefaciens of the expression product of binary vector with the gene for the cecropin A-mellitin hybrids synthesis in plant cells. pp. 55--58
Summary: On the basis of binary vectors pBI121 and pBIDGUS the synthetic genes have been obtained to express the hybrid cecropin A-melittin antimicrobial peptides CEMA and MsR1 in plant cells. As a result of these vectors cloning the products of the expression of pBIDGUS-based vector are found to be hazardous for the cells of E. coli and A. tumefaciens.
Abramchik L. M., Serdjuchenko E. V., Zenevich L. A., Kabashnikova L. F.
Influence of hight intensity light with heat stress on structural and functional state of barley photosynthetic membranes (Hordeum vulgaris L.). pp. 59--62
Summary: The influence of high intensity light on photosynthetic apparatus state and the role of high temperature in forming of light stability mechanism in green barley leaves were studied. The increase of the lipid peroxidation level under high light was revealed. The content of finish peroxide prodacts in membrane decreased up to the control meaning when green seedlings had been lit after termotreatment (40°C, 3h). PS 2 functional activity in plants was limited by high intensity light. Photochemical quenching (qP) decreased and nonphotochemical quenching (qN), which characterized thermal dissipation level of the absorbed quantes, increased in this condition. Heat treatment, which took place before the high light effect, made up the correlation of intensitives of low temperature fluorescence (I740/I685) to the control, parameters of photochemical and nonphotochemical quenching corresponded to the control too. Obtained data demonstrated that preliminary termotreatment before high intensive light effect prevents destruction processes in photosynthetic membranes.
Kozel N. V., Shalygo N. V.
The peroxidation of lipids and permeability of cellular membranes in barley leaves under photooxidative stress induced by Rose Bengal. pp. 63--66
Summary: Accumulation of TBA-products and efflux of electrolytes and free nucleotides from cells in green barley leaves during photooxidative stress induced by the Rose Bengal photosensitizer were found. These results indicated that processes of the lipid peroxidation and breaking of barrier properties of cellular membranes are increased.
Mikhailova R. V., Zhukouskaya L. A, Semashko T. V., Paulovskaya Zh. I., Chikhaeva O. V., Lobanok A. G, Kartel A. N.
Effect of long-term preservation conditions on viability of fungus Penicillium adametzii LF F-2044.1 and synthesis of glucose oxidase. pp. 67--71
Summary: These were performed to evaluate effect of long-term preservation methods on viability of fungus Penicillium adametzii LF F-2044.1 and stability of its capacity to synthesize glucose oxidase. It was found that optimal methods for long-term storage of the fungus are periodic transfers on wort-agar media with 1.5% NaCl and cryoconservation using 5% DMSO as cryopro-tector ensuring 100% survival of the fungus and enhanced level of glucose oxidase production.
Makovich O. M., Mikhailova R. V., Lobanok A. G.
Influence of nutrient medium components on growth of Phellinus robustus K. and extracellular peroxidases biosynthesis. pp. 72--78
Summary: Influence of nutrient medium components on growth of wood-degrading basidiomycete Phellinus robustus K and biosynthesis of extracellular peroxidase was studied. It was established that the fungus showed good growth using glucose, fructose, xylose, pectin, starch, lignin and cellulose as a source of carbon and asparagine, glutamic acid, urea and yeast extract as a nitrogen source. Ph. robustus K did not utilize citric and oxalic acids, tannin, carboxymethylcellulose, potassium nitrate, ammonium nitrate and ammonium sulfate. It was found that maximum level of peroxidase synthesis was observed on the medium with 0.5--1% glucose as the sole source of carbon and 0.3% asparagine or 0.045% urea as the only source of nitrogen, and also during combined use of glucose + cellulose and glucose + lignin in composition of the nutrient medium. Peroxidase production was not induced by rhamose, all tested alcohols and carboxylic acids, aspartic and glutamic acids. Correlation of peroxidase and laccase forms produced by Ph. robustus K with carbon and nitrogen sources of nutrition was demonstrated.
Lelevich S. V.
Action of morphine intoxication on the endocrine function of the rat thyroid gland. pp. 79--82
Summary: The study on the endocrine activity of the rat thyroid gland was carried out under acute and chronic morphine intoxication. The serum levels of T4, T3, tireotropine and tiroxinebond globuline were assayed by mean of radioimmune methods. It was showh that acute morphine administration led to the dose- dependent increase in the hormones levels. Prolongation morphine administration conducted to decrease in the hormone contents 2 and 3 weeks after morphine intoxication.
Sutsko I. P.
Investigation of hepatoprotective action of leucovorine under acute tetrachlormethane intoxication. pp. 83--87
Summary: It was shown acute tetrachlormethane intoxication made up 48% death of rats, increasing contents of middle molecular peptides and formation of thymolum-lipide complex, activity g-gluthamiletranspeptide in the blood and falling xenobiotic metabolizing liver function. Administration of leucovorine reduced animal mortality by two times, normalized content middle molecular peptides in the blood, and also oxidation of NADH, NADPH and NADH-, NADPD-ferricianide reductases in microsomal fraction of rat liver.
Nikitin I. G., Beloenko E. P. †, Pashkevich L. A., Grits A. N., Karalamak A. I., Veremey E. I.
Comparative and experimental analysis of osteoregeneration among rodents under the influence the suspension of osteoblasts a man and amphibian. pp. 88--94
Summary: The same results were obtained by possible cross-stimulating processes of repairing osteoregeneration in different kind of animals (chordata) with the help of adding warm- and cold-blooded osteoblast is culture. It shows the presence of osteoblast's culture, differentiation and proliferation mechanisms by meams receptor apparatus of mesenchymal cells cytolemma predecessor antecedent and osteoblast themselves. The presence of local inflammation reaction when introducing human embryonal osteoblast suspensions culture and determination of similar neoosteogenesis visual-spectrographic process in the site of mechanism osteoblast differentiation regulation, prolifiration and it is possible the immune system took put participation in this process. The obtained dealing with availability of similar albumin fractions by molecular mass size at different classes of mammals and amphibians that were received by electroforetic division of extracts bone tissues and culture osteoblasts of different animal classes was shown the possibility of neoosteogenesis stimulation through cytokine-like receptor-regulating peptides from cytolemming apparatus of osteogenes cells or by means introduction of osteogenes cell suspension and their predecessors of embryo from one and different kind of animals.
Vergeichik L. A., Kozulin A. V., Grichik V. V., Minets M. L., Pareiko O. A.
Breeding ecology of the aquatic warbler Acrocephalus paludicola on the Dicoe fen mire in the Yaselda river head. pp. 95--101
Summary: The breeding ecology of aquatic warbler on the Dikoe fen mire, which is one of the most important habitats of this globally threatened species in Europe was studied. The breeding success (the probability of success from the start of incubation to fledging, Mayfield method) varied from year to year within the range of 36.3% through 2.89% (28 nests). The main cause of the low breeding success is predation by shrews (the daily egg mortality rate due to predation is 2.5% and the daily nestling predation rate -- 5.04%) has been discovered. High density of shrews at mesotrophic fen mires may be one of the factors limiting the abundance of the species was suggested.
Baitchorov V. M., Hihiniak Yu. G.
The glacial relic fauna of the lake Yuzhny Volos. pp. 102--106
Summary: The estimation of a modern state and comparative analysis of the population structure of glacial benthic and planktonic species of the lake Yuzhny Volos has been done. It is shown that the lake is a water-body alone in Belarus with a complete set of glacial relic species Mysis relicta, Pontoporeia affinis, Pallasea quadrispinosa, Limnocalanus macrurus and a single one where the first of them is found. First in Belarus the relic mollusc species Pisidium conventus in this lake has been revealed. It is noted that the restoration of the population of noble crayfish entered the Red Data Book of Belarus is taking place in the lake. Obviously the lake Yuzhny Volos may be the most significant water-body in Belarus in respect to its unique biological diversity.
Rotenko I. I., Sidorovich V. E.
Diet and habitat distribution of the weasel Mustela nivalis in natural complex Nalibokskaya Pyscha (test of selectivity). pp. 107--111
Summary: Radio tracking of two weasels Mustela nivalis was carried out in order to study the selectivity in populating different bi-otopes on the territory of natural complex Nalibokskaya Puscha. At the same time the weasel diets were studied. During the research it was revealed that forest biotopes (spruce and alder forests) and their ecotones with open biotopes were the main habitats of the weasels. It was revealed that the radiotracked weasels (both male and female) mostly preferred feeding on Microtus voles. Bank voles being the predominant species among small rodents were also consumed on a pretty large scale but less than Microtus voles.
Derunkov A. V.
Species composition and dominant structure of the rove beetle assemblages (Coleoptera, Staphylinidae) in different biocenoses in the Mid-Pripyat National Landscape zakaznik. pp. 112--119
Summary: The rove beetles in the different biocenoses in the Mid-Pripyat National Landscape zakaznik were collected by means of pitfall traps. Totally of 135 rove beetle species were found out, 71 of they -- in forests, 88 -- in open biocenoses. The dominant structure of rove beetle assemblages was studied. It was ascertained that species composition and dominant structure of the rove beetle assemblages is closer in the forest biocenoses than in the open biocenoses. It is presented that biocenoses in zakaznik have a great value for protection of the beetle assemblages inhabiting the soil surface.
SCIENTISTS OF BELARUS
Anatoly Georgievich Lobanok (To the 70th Anniversary). pp. 120--121
Click here to read this article in Russian (Win1251)
Eleonora Ivanovna Khotko (For her Jubilee). pp. 122--123
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